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KMID : 1025520040460040537
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Journal of Animal Science and Technology
2004 Volume.46 No. 4 p.537 ~ p.546
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Chromosomal Localization and Mutation Detection of the Porcine APM1 Gene Encoding Adiponectin
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Park Eung-Woo
Kim Jae-Hwan
Seo Bo-Yeong
Jung Kie-Chul
Yu Seong-Lan
Cho In-Cheol
Lee Jung-Gyu
Oh S.-J.
Jeon Jin-Tae
Lee Jun-Heon
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Abstract
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Adiponectin is adipocyte complement-related protein which is highly specialized to play important roles in metabolic and hormonal processes. This protein, called GBP-28, AdipoQ, and Acrp30, is encoded by the adipose most abundant gene transcript 1 (APM1) which locates on human chromosome 3q27 and mouse chromosome 16. In order to determine chromosomal localization of the porcine APM1, we carried out PCR analysis using somatic cell hybrid panel as well as porcine whole genome radiation hybrid (RH) panel. The result showed that the porcine APM1 located on chromosome 13q41 or 13q46-49. These locations were further investigated with the two point analysis of RH panel, revealed the most significant linked marker (LOD score 20.29) being SIAT1 (8 cRs away), where the fat-related QTL located. From the SSCP analysis of APM1 using 8 pig breeds, two distinct SSCP types were detected from Korean native and Korean wild pigs. The determined sequences in Korean native and Korean wild pigs showed that two nucleotide positions (T672C and C705G) were substituted. The primary sequence of the porcine APM1 has 79 to 87% identity with those of human, mouse, and bovine APM1. The domain structures of the porcine APM1 such as signal sequence, hypervariable region, collagenous region, and globular domain are also similar to those of mammalian genes.
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KEYWORD
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Pig, APM1, Mapping, SSCP, Mutation
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